ProtaGene CGT GmbH

Gene therapy approaches are increasingly used for the treatment of life-threatening human diseases. The GeneWerk GmbH, a spin-off of the German Cancer Research Center in Heidelberg and founded in July 2014 by Prof. Christof von Kalle, Dr. Manfred Schmidt und Dr. Annette Deichmann offers custom tailored service based on 20 years of experience in the field of gene therapy, gene editing, immunotherapy and related areas. The team has long-lasting experience in the area of hematology, oncology and virology with focus on integration site analysis, high throughput sequencing, whole genome sequencing and bioinformatics.
The service concerns the experimental work and particularly the bioinformatical analysis of the data. The data of the customers do not only run through GeneWerk’s standard analysis pipelines but additionally they will be analyzed intensively by the highly qualified team members.

Analyses and services GeneWerk can provide:
• Integration site analysis of viral vectors with (nr)LAM-PCR – The standard
analysis 

• Integration site analysis of viral vectors with Target Enrichment Sequencing
(TES): This new method will provide the results much faster and more
reliable. 

• Gene editing on-/off- target integration analysis 

• Immune repertoire analyses by sequencing the hypervariable region of 
 the T- cell receptors to characterize the clonality and the functional 
 status of a T-cell population 

• Quantitative PCR (qPCR) for subsequent amplification and quantification 
 of DNA and RNA fragments 

• Gene expression analyses 

• Development of bioinformatics analysis tools for next-generation- 
 sequencing-projects 


Further information on the websites of BIOPRO Baden-Württemberg: